Journal: Dose-Response
Article Title: Stachydrine Alleviates Sepsis-Induced Cardiomyopathy by Inhibiting Ferroptosis via Regulating SIRT1/GPX4 Pathway
doi: 10.1177/15593258251377710
Figure Lengend Snippet: STA Suppresses Myocardium Apoptosis and Injury in LPS-Treated Mice. (A) Apoptosis was Evaluated by TUNEL Staining in the Myocardium (Magnification 200×). (B) Quantification of TUNEL-Positive Cells. Spectrophotometer and ELISA Were Used to Measure Serum Levels of Myocardial Injury-Related Molecules, including (C) CK-MB, (D) LDH, (E) BNP, (F) cTnI, and (G) cTnT. Data are Presented as Mean ± SD (n = 8 Mice in Each Group) and Analyzed Using One-Way ANOVA, With the Bonferroni Test Employed for the Post Hoc Analysis. *** P < 0.001 vs Control Group; ### P < 0.001 vs LPS Group. CK-MB, Creatine Kinase Isoenzyme MB; LDH, Lactate Dehydrogenase; BNP, Brain Natriuretic Peptide; cTnI, Cardiac Troponin I; cTnT, Cardiac Troponin T
Article Snippet: The quantification of several biomarkers, including creatine kinase MB (CK-MB) (SEKM-0152, Solarbio, China), brain natriuretic peptide (BNP) (E-EL-M0204c, Elabscience, China), cardiac troponin I (cTnI) (SEKM-0153, Solarbio, China), cardiac troponin T (cTnT) (SEKM-0150, Solarbio, China), interleukin-1β (IL-1β, MLB00 C, R&D Systems), interleukin-6 (IL-6, M6000 B, R&D Systems), and tumor necrosis factor-alpha (TNF-α, MTA00 B, R&D Systems) in blood serum samples was performed using ELISA assay kits.
Techniques: TUNEL Assay, Staining, Spectrophotometry, Enzyme-linked Immunosorbent Assay, Control